Journal of Environmental Treatment Techniques  
2020, Volume 8, Issue 3, Pages: 1081-1083  
J. Environ. Treat. Tech.  
ISSN: 2309-1185  
Journal web link:  
Formulation of Microorganisms for Cane Plants  
Ririen Prihandarini* and Sudiarso  
Lecturer at the Faculty of Agriculture, University of Widyagama Malang, Indonesia  
Received: 25/03/2020  
Accepted: 29/06/2020  
Published: 20/09/2020  
Sugar is one of the strategic commodities in the Indonesian economy. With an area of around 450 thousand hectares in the 2007-  
017 period, the sugar cane-based sugar industry is one of the sources of income for around 900 thousand farmers with the number of  
workers involved reaching around 1.3 million people. Sugar is also one of the basic needs of the community; the dynamics of sugar  
prices will have a direct influence on the inflation rate. One effort that can be done to improve sugarcane productivity is to improve  
soil fertility through sugarcane cultivation techniques by engineering microorganism technology. The potential of nature and the  
wealth of microorganisms in Indonesia are numerous but have not been explored and utilized to the full Engineering the use of  
microorganisms has good prospects for restoring land degradation in sugarcane agroecosystem. Microorganism-formulation was  
designed to get the most suitable microorganism combination for sugarcane. Various types of Nitrogen-fixing microorganisms such as  
Nitrobacter, Nitrosomonas, Nitrosococcus, and Rhizobium will be combined with Phosphate solvent bacteria such as Pseudomonas,  
Bacillus, Pseudomonas, Xanthomonas, Acetobacter Thiobacillus, Thrichodema and some decomposer bacteria. Found the most  
suitable formulation for the growth of sugarcane seedlings, formulation of the microorganisms Sacharomyces (S), Azospirillum sp (A),  
Azotobacter (Z), Rhizobium (R), Pseudomonas (P), Bacillus dan Trichoderma sp (T) which is formulated in a microorganism  
technology with the popular name Refresh Microorganism (RIM).  
Keywords: Cane, Formulations, Microorganisms  
provide nutrients according to plant requirements have been  
obtained [4], only still need to be modified specifically for  
sugarcane. The soil health index of Indonesia must also be  
considered for better plant requirements achieved [5-7]. The  
purpose of this study is to formulate a consortium of  
microorganisms that can help sugarcane plants to grow better.  
Dependence on chemical fertilizers causes chemical  
fertilizer subsidies to increase every year. Environmental  
burden caused by the use of chemical fertilizers continuously  
in high doses increasingly close the pores of the soil, so that  
the absorption of rainwater is greatly decreased and resulting  
in frequent floods and drought [1]. In addition, the use of  
chemical fertilizers continuously causes the soil to become  
hard so that the main crop production of sugar cane becomes  
smaller and the yield of sugar obtained is low [2]. It is time  
for our farming system to return to nature with organic  
farming. Microorganism technology will help organic farming  
systems to be realized easily, quality and inexpensive so it  
must be prepared on an industrial scale [3].  
Synergy between microorganism technologies intended  
for the development of organic agriculture and sugar cane  
farmers as users is something that needs to be socialized and  
implemented in the field. For this reason, continuous research  
is needed. The potential of microorganisms in Indonesia is  
extraordinary in terms of species, quantity and function; this is  
due to the fact that in Indonesia, 2 of these mountain ranges in  
the world meet, in Indonesia located between 3 continents and  
the meeting of the Indian and Pacific oceans.  
2 Materials and Methods  
This research was conducted from April in the Laboratory  
of the Department of Agrotechnology, Faculty of Agriculture,  
Brawijaya University Malang. This formulation research was  
carried out by mixing various pure cultures of photosynthetic  
bacteria Sacharomyces (S), Azospirillum sp (A), Azotobacter  
(Z), Rhizobium (R), Pseudomonas (P), Bacillus (B) and  
Trichoderma sp (T) with natural ingredients such as banana  
weevil and sugar cane shoots that contain a variety of natural  
hormones. The formulation trials are used on sugarcane  
seedlings, the seeds that grow best will be found with the  
fastest growing indicator of shoots.  
Results and Discussion  
Based on the synergy test between a consortium of  
This has the opportunity to build a microorganism  
industry that has the opportunity to increase crop production  
and quality, especially sugar cane. Reliable pioneer  
microorganisms that have been selected by the research team  
since the 1990s in this study combined into a force that can  
help sugarcane plants to produce high yields with high yields.  
Pioneering microorganism formulations that are reliable  
in improving polluted environments and helping plants  
Sacharomyces (S), Azospirillum sp (A), Azotobacter (Z),  
Rhizobium (R), Pseudomonas (P), Bacillus (B) and  
Trichoderma sp (T) with a comparison, the formula obtained  
F1, F2, F3, F4 and F5 that are used can synergize with one  
another. From the results of incubation for 24 hours, each  
formula contained dominance and which could blend (Table  
.1 Antagonist  
Bacterial consortium is a collection of bacteria that work  
together to form a community, to produce significant  
Corresponding author: Ririen Prihandarini, Lecturer at the  
Faculty of Agriculture, University of Widyagama Malang,  
Indonesia. E-mail: